Pesticides have been widely used in the U.S. for decades to combat everything from weeds to insects to bacteria. These compounds allow farmers to cultivate acres of successful crops and keep food on our dinner tables. But every chemical poses a risk, so I always like to familiarize myself with the chemicals I’m being exposed to, in order to make informed decisions about the health and safety of me and my family. Here are a few facts about pesticides I thought I’d share – just in case you’re on the same fact-finding journey I am.
If you’re reading this blog and hoping for a sneak peek at the list of contaminants that will be on the next UCMR list, you’ll want to keep reading…
Have you ever stopped to enjoy a bright, vibrant sunset, only to have that really annoying friend interrupt your thoughts with a comment like “you know you’re just looking at all the pollution in the air, right?”
I used to wonder how someone could focus on pollution while looking at a stunning landscape, but it’s becoming a topic that more and more people are thinking about.
“Why do I keep seeing background contamination from phthalate and adipate when I do extractions for semi-volatiles?”
This is one of the most common questions I’ve been asked when I’m traveling in the field. It’s an issue I’ve come across in my own lab on occasion and if you can’t find the source of your contamination, it can turn routine application work into a troubleshooting nightmare. Given how often I’ve seen these compounds cause contamination issues, I thought I’d review some of the most common sources for these. Continue reading 5 Sources of Phthalate and Adipate Contamination You Probably Didn’t Know About
In the first part of this 2-part blog series, I highlighted the improvements made by the EPA regarding the preparation and preservation of samples. In this post, I will focus more on the changes the EPA has made to Method 525 which affect the analysis of the prepared samples.
Have you ever wondered why solution flow rates are so important when performing sample preparation with solid phase extraction (SPE)? If you have, read on – I have the answer for you!
Throughout my college career, the phrase “like dissolves like” was referred to quite frequently. This phrase was particularly relevant when we did solubility experiments and for good reason – it’s 100% true! Solvents tend to dissolve solutes with physical and chemical properties that are similar to theirs. Other factors such as temperature, pressure and pH can affect the solubility of solutes as well, but let’s just keep it simple for the purposes of this discussion and keep it focused on physical and chemical properties. Given this simplistic definition of solubility, the opposite stands true as well – solutes don’t tend to dissolve into solvents with differing physical and chemical properties. These solvents and solutes want to stay as far from each other as is possible.
There’s nothing more satisfying than successfully extracting a really challenging sample. Solid phase extraction (SPE) is a powerful technique for extracting semi-volatile organic compounds and hexane-extractable materials (HEMs). When the chemistry is tailored to meet the requirements of the application, literally hundreds of compounds can be extracted with a single pass of solution through an SPE disk.
Phenolic compounds can be some of the most challenging compounds to extract from the compound lists in EPA Method 8270 and EPA Method 625.1. The recovery of these compounds suffer tremendously compared to some of the other target analytes on the list. So what exactly are phenols and why are they challenging to extract and quantitate?
“I’m so tired of doing dishes!”
Between the dishes I wash at home and those I wash in the lab, that phrase leaves my lips no fewer than 3 times a day. If I were to add up the number of hours I’ve spent washing dishes over the past year, I’d….well…it’s too upsetting, so I try not to do that calculation. Let’s just say I’d have had time to become a seasoned marathon runner and to backpack across both Europe and parts of Australia.
If you’re a laboratory that’s processing drinking water samples using solid phase extraction, you’ve inevitably gotten to the step in your procedure where you’ve eluted your analytes from your SPE media and you find yourself saying “How do I dry my extracts?”
What’s the best way to dry my extracts?
This is a question we get quite frequently and it’s a reasonable question to ask. Unfortunately, the answer is – it depends. Solvent drying (not to be confused with solvent evaporation) is an important step in your extraction process when you’re using organic solvents to elute your target analytes. Residual water in your solvent can cause issues if your target analytes extract back out of the solvent and into the water while you’re trying to evaporate or analyze your sample. Water can also damage your chromatography system, so if you’re quantifying your extracts by GC/MS or LC/MS, you want your solvent extracts to be dry prior to analysis.
So, given the importance in solvent drying, I thought I’d share some of the commonly asked questions that come up under this topic.
Q: I’m processing my samples against EPA Method 525.3. Does it matter how I dry my extracts?
A: If your lab is being audited against EPA Method 525.3, you need to dry your extracts per the recommended procedure – in this case, sodium sulfate. Make sure you purchase the recommended grade of anhydrous sulfate and store it appropriately.
If your lab processes a large volume of samples, you may have sought out alternative approaches to solvent drying, such as phase separation membrane. While sodium sulfate is readily available for purchase in bulk quantities and is pretty easy to learn how to use, it has some potential downsides to it.
- It has to be dried and carefully stored, which is time-consuming and requires you to have adequate drying and storage equipment
- It has to be disposed of as hazardous waste
- It’s a chemical that dries your solvent by reacting with water to form a hydrated salt, which means it can retain some of your target compounds (particularly those that are highly water soluble)
- It can contaminate your extract, particularly if it’s stored incorrectly or purchased at a lower grade than is recommended
- It can be saturated. What that means is, if you didn’t calculate the mass of sodium sulfate you needed, given the volume of water you needed to remove, you could exceed the capacity of the salt and end up with a solvent that’s not completely dry
Phase separation membranes physically separate the water from your solvent, which eliminates all of the challenges you face with a chemical drying agent such as sodium sulfate. Plus, it’s compact and easy to store, intuitive to use and easy to dispose of.
There are a handful of benefits to using a phase separation membrane over sodium sulfate – just make sure you check the method you’re following and adhere to the drying method outlined there (if there is one). Check out the method summary in this app note for an example protocol that adheres to EPA Method 525.3 guidelines.
Q: Since EPA Method 525.3 specifies that I use sodium sulfate, can I put sodium sulfate on top of phase separation membrane to dry my extracts?
A: While clever, this is an idea that you would want to run past your auditor first. Since the method specifies the use of sodium sulfate but does not specify the physical separation of water (using a phase separation membrane, for example), physical separation isn’t forbidden, but it’s also not specifically allowed. Yep, this one is a gray area so have a conversation with your auditor before cleverly devising a drying setup that includes both chemical and physical solvent drying.
Q: I’m running samples against EPA Method 525.2. Do the same rules apply to me?
A: Yes. As with Method 525.3, this method specifies the use of sodium sulfate.
Q: I’m not processing samples against an EPA Regulated Method and my protocol doesn’t specify a protocol for extract drying. What should I do?
A: If your lab is not reporting results against a method that specifies an extract drying method, you should have the option to decide whether you want to dry your extracts using physical or chemical separation (double check your laboratory’s established protocols to make sure your SOP allows you this flexibility).
If this decision were up to me, I’d order myself a huge stack of DryDisk® Disks and wave goodbye to sodium sulfate forever!
Do you prefer physical drying over chemical drying? If so, let us know in the comments and share this post to spread the word!